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More about AUG start codon recognition by bacterial ribosomes

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Book synopsis

Initiation is the rate-limiting step of translation and is an important regulatory step in gene expression. Translation initiation requires ribosome recognition of the mRNA s start codon followed by formation of a stable ternary complex involving initiator tRNA. Studies have shown specific contacts between a AUG start codon and several ribosomal proteins (r-proteins). We employed short-leadered mRNAs in order to further characterize the path of an mRNA s 5 -AUG from initial association with the ribosome to its final placement at the ribosome s decoding site (P-site). Our results suggest that bacterial ribosomes can bind and inspect the 5 -terminus of all mRNAs for an AUG triplet, with close proximity of AUG to the 5 -terminus positively influencing ribosome binding and ternary initiation complex formation. Crosslinking studies showed that while 5 -AUG interactions with r-proteins S1, S3, S4, S7, S10 and S18 can occur in the absence of tRNA-fMet, movement of a 5 -AUG into the Psite requires tRNA-fMet.

Book details

Book category Books in English Mathematics & science Biology, life sciences Microbiology (non-medical)

• Full title: AUG start codon recognition by bacterial ribosomes
• Author: Karthik Krishnan
• Language: English
• Binding: Paperback
• Number of pages: 148
• EAN: 9783639511895
• ISBN: 3639511891
• ID: 07170230
• Publisher: Scholars' Press
• Weight: 227 g
• Dimensions: 229 × 152 × 9 mm
• Date of publishing: 13. February 2013

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