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Book synopsis

This volume details the fundamentals of the CRISPR-Cas system , and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

Book details

Book category Books in English Mathematics & science Biology, life sciences Life sciences: general issues

• Full title: CRISPR-Cas Methods
• Author: M. Tofazzal Islam, Pankaj Bhowmik, Kutubuddin Ali Molla
• Language: English
• Binding: Hardback
• Number of pages: 278
• EAN: 9781071606155
• ID: 25603516
• Publisher: Springer-Verlag New York Inc.
• Weight: 758 g
• Dimensions: 254 × 178 × 22 mm
• Date of publishing: 30. June 2020

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