You might also like

More about Microrna Processing in Arabidopsis Thaliana

• Book details
• Synopsis
• Trending among others

You get 223 loyalty points

Book synopsis

Plant microRNAs (miRNAs) are processed by the RNase III-like enzyme DICER-LIKE1, the double-stranded RNA-binding protein HYPONASTIC LEAVES1 and the zinc finger protein SERRATE. Together, they excise a miRNA/miRNA duplex with a 2 nucleotide 3' overhang from the primary miRNA (pri-miRNA) transcript. pri-miRNAs include a partially self-complementary foldback, which gives rise to the mature miRNA. In animals, pri-miRNAs are very similar, with a stereotypic position of the miRNA within the foldback. Accordingly, rules for miRNA excision from the precursor are quite simple in animals. In contrast, how miRNA sequences are recognized in the structurally much more diverse foldbacks of plants is unknown. We have performed an extensive in vivo structure-function analysis of Arabidopsis thaliana pri-miRNA 172a. A junction of single-stranded and double-stranded RNA 15 nucleotides proximal from the miRNA duplex appears to be essential for accurate miR172a processing. This attribute is found in several other but not all plant miRNA foldbacks. In addition, we engineered de novo a functional minimal miRNA precursor.

Book details

Book category Books in English Mathematics & science Biology, life sciences

• Full title: Microrna Processing in Arabidopsis Thaliana
• Author: Schallum Werner
• Language: English
• Binding: Paperback
• Number of pages: 136
• EAN: 9783838120188
• ISBN: 3838120183
• ID: 07013460
• Publisher: Sudwestdeutscher Verlag Fur Hochschulschriften AG
• Weight: 209 g
• Dimensions: 229 × 152 × 8 mm
• Date of publishing: 10. September 2010

Trending among others

---

---

---