Code: 06832786

Characterizing Osteoblast Mineralization Using Ftir

Name: Characterizing Osteoblast Mineralization Using Ftir
Brand: VDM Verlag
SKU: 06832786
Price: 49.83 EUR
Availability: BackOrder
Author: Alka Potdar
ISBN: 9783639253030

by Alka Potdar

Language: English
Binding: Paperback
Number of pages: 116
Publisher: VDM Verlag, 2010
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The ability of a cholesterol-reducing drug, simvastatin, to stimulate the formation of hydroxyapatite in an osteoblast-like cell line, was studied using Fourier transform infrared spectroscopy. Hydroxyapatite was detected using both Fourier transform infrared spectroscopy and von Kossa staining techniques a week earlier when cells were cultured in growth media supplemented with simvastatin in a tissue culture polystyrene system. Analysis of infrared spectra using area integration and curve-fitting techniques was performed to quantify the hydroxyapatite content in cells relative to the biological matrix. Infrared spectroscopy was used to quantify the mineralization process. Cells grown in a rotating bioreactor mineralized more rapidly than cells grown in tissue culture polystyrene systems.