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Book synopsis

Regarding a strategy of expressing foreign genes in§plants, two diverged RMV-based expression vectors§containing a green fluorescent protein were cloned§into the binary vector pPZP200 for subsequent§agroinfiltration work. An infectious full-length§clone of RMV and three RMV-based mutants already§existed. They vary depending on which of the two§putative ORFs is inactivated. An additional mutation§site of one of these mutation constructs was§discovered and reverse mutated. After the inoculation§of the constructs on N. benthamiana and N. tabacum,§the analysis revealed distinct effects in virus§accumulation and symptom characteristics of the three§mutation constructs. These results differ from the§ones of the wild type clone. Accordingly, all three§mutants show both lower virus accumulation levels and§lesser symptom characteristics. Further, the results§from N. benthamiana indicate an ability to reverse§mutate the -4K virus. In addition to the description§and the analysis of these results, the potential role§of the two putative ORFs will be discussed and an§outlook on further investigations will be given.

Book details

Book category Knihy po anglicky Medicine Other branches of medicine Pathology

• Full title: Molecular Characterisation of the Ribgrass Mosaic
• Author: Pierre Hohmann
• Language: English
• Binding: Paperback
• Number of pages: 112
• EAN: 9783639090284
• ISBN: 3639090284
• ID: 06818134
• Publisher: VDM Verlag Dr. Mueller E.K.
• Weight: 159 g
• Dimensions: 229 × 152 × 6 mm
• Date of publishing: 06. October 2008

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