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Viac informácií o knihe Synthetic Green Fluorescent Protein Chromophores

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GFP is widely used as a §biological marker because of its superb fluorescence §properties. We have synthesized GFP chromophore and §its derivatives, and their photochemistry §and photophysics were investigated using various §steady-state and time-resolved techniques. §We first compared their optical properties between §solution and solid state. We found some O-alkyl §GFP chromophore derivatives exhibit large §fluorescent enhancement in the solid state.§Cis/trans isomerization is thought to be responsible §for blinking behavior in fluorescent protein; §however, the mechanism of the thermal reverse §isomerization is more problematic. Using §Hammett plot studies, we conceived, §for the first time, a novel nucleophilic §addition/elimination mechanism. §Finally, a hydroxyl substituent at the meta position §shows enhanced charge transfer and greater acidity §in the excited state. As a result, we have §demonstrated that the fast quenching of the excited §state by internal conversion to the ground state is §much slower in meta- than in para-HOBDI derivatives. §This allows studies of this ultrafast intermolecular §ESPT that competes with isomerization.

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Zaradenie knihy Knihy po anglicky Mathematics & science Chemistry Organic chemistry

• Celý názov: Synthetic Green Fluorescent Protein Chromophores
• Podnázov: PHOTOCHEMICAL AND PHOTOPHYSICAL STUDIES OF SYNTHETIC DERIVATIVES OF THE GREEN FLUORESCENT PROTEIN CHROMOPHORE
• Autor: Jian Dong
• Jazyk: Angličtina
• Väzba: Brožovaná
• Počet strán: 128
• EAN: 9783639134537
• ISBN: 3639134532
• ID: 06822152
• Nakladateľ: VDM Verlag
• Hmotnosť: 200 g
• Rozmery: 229 × 152 × 8 mm
• Dátum vydania: 01. April 2009

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