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Viac informácií o knihe E. coli Plasmid Vectors

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Nákupom získate 623 bodov

Anotácia knihy

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results. §Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.

Parametre knihy

Zaradenie knihy Knihy po anglicky Mathematics & science Biology, life sciences Life sciences: general issues

• Celý názov: E. coli Plasmid Vectors
• Podnázov: Methods and Applications
• Autor: Nicola Casali, Andrew Preston
• Jazyk: Angličtina
• Väzba: Pevná
• Počet strán: 316
• EAN: 9781588291516
• ISBN: 1588291510
• ID: 01430251
• Nakladateľ: Humana Press Inc.
• Hmotnosť: 628 g
• Rozmery: 159 × 243 × 24 mm
• Dátum vydania: 03. July 2003

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