Kód: 06812390
Aim of this study was to select environmental E. coliisolates that produced colicins against the swine pathogen E. coliK88+. In initial evaluation using a modified plate method with 18colicinogenic E. coli constructs, colicins E3, ... celý popis
Nákupom získate 157 bodov
Aim of this study was to select environmental E. coliisolates that produced colicins against the swine pathogen E. coliK88+. In initial evaluation using a modified plate method with 18colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, Kand N were found to possess inhibitory activity against 12 ETECK88+ strains. A total of 463 environmental isolates from cattlerumen, cattle feces, pig feces and hog manure-amended soil werescreened for colicin production by a modified plate test.Further,colicinogenic isolates were screened for five toxin genesLT, STa, STb, VT1 and VT2 as well as K88 (F4) fimbriae using PCRreactions. Fourteen non-pathogenic isolates were subjected tocharacterization of colicin genes by PCR using 9 new primersequences, antibiotic susceptibilities and substrate utilization.Two potential probiotic strains of E. coli, UM-2 and UM-7 whichproduced colicins that could utilize potato starch and inulin wereselected for in-vitro competition with E. coli K88+ strain 2-12. Invitro competition between the synbiotics and E. coli K88+ revealedinhibition of E. coli K88+. Animal testing of these strains iscurrently being undertaken.
Zaradenie knihy Knihy po anglicky Medicine Veterinary medicine
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