You might also like

More about Gene Knockout Protocols

• Book details
• Synopsis
• Trending among others

You get 345 loyalty points

Book synopsis

Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered.§State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

Book details

Book category Knihy po anglicky Mathematics & science Biology, life sciences Life sciences: general issues

• Full title: Gene Knockout Protocols
• Author: Martin J. Tymms, Ismail Kola
• Language: English
• Binding: Hardback
• Number of pages: 431
• EAN: 9780896035720
• ISBN: 0896035727
• ID: 01400771
• Publisher: Humana Press Inc.
• Weight: 826 g
• Dimensions: 229 × 152 × 29 mm
• Date of publishing: 15. January 2001

Trending among others

---

---

---