Preparation and characterization of new HPLC stationary phase / Najlacnejšie knihy
Preparation and characterization of new HPLC stationary phase

Kód: 07161435

Preparation and characterization of new HPLC stationary phase

Autor Emad Yousif, Hajer Saad Najem, Shahbaz Maki

New stationary phase for high performance liquid chromatography had been prepared by the reaction of congo red solution with amberlite anion exchange. The F.T.I.R elemental analyses have configured the attachment of congo red on t ... celý popis

63.70


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Viac informácií o knihe Preparation and characterization of new HPLC stationary phase

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Anotácia knihy

New stationary phase for high performance liquid chromatography had been prepared by the reaction of congo red solution with amberlite anion exchange. The F.T.I.R elemental analyses have configured the attachment of congo red on the resin as has indicated by the physical appearance of the new resin. The resin capacities of newly prepared resin has calculated the resin capacity for the amberlite-congo red resin was found to average 4.8 meq/ gm and for the amberlite resin 3.0 meq./gm. The new stationary phase has been packed into stainless steel column. This has been done by the special teqnique. The chromatographic performance of the packed column was characterized the number of plate numbers, height equivalent of a theoretical plates, capacity factors, selectivity factor and peak asymmetry were measured by analyzing different analysis on the new column using different mobile phases composition and flow rates. The parameters were measured from analasisi of some amino acids such as histidine, phenyl alanine, tyrosine and prolenene. The analysis of amino acids using amberlite-congo red column has performed with an aqueous mobile phase adjusted at pH 2.5.

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63.70

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